Electrophoretic separation of humic acids obtained from soil was attempted through denaturing polyacrylamide gel electrophoresis (dn-PAGE) by modifying the known methods. The modifications of sample preparation and gel composition led to significant improvement in both visibility and intensity of humic acid profile. The comparative profile showed a similar nature of humic acid components in all the samples and the commercial standard. The Fourier transform infrared spectroscopy (FTIR) spectra indicated the presence of carboxyl and carbonyl functional groups and aliphatic chains in humic acid samples. The modified method facilitated better separation of humic acid. The results of the present study indicate the potential of dn-PAGE for electrophoretic separation of different components of humic acids.

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